Competition 1 site

Formula

Y=Par1*Par2/(Par2+X)+Par3

Parameters

Par1=B₀ amount bound in the absence of competing drug
Par2=IC₅₀ amount of competing drug inhibiting 50% of labeled binding
Par3=NSB unspecific binding

Parameter limits

Minimum=10^-30
Maximum=10⁺³⁰

Data entry

Enter displacer concentrations in first column and bound values in the total bound column(s). Unspecific binding is calculated by the model

Initial estimates

Can be generated automatically or entered manually. Automatic estimates are obtained by linear transformation.