Dissociation 1 site

Formula

Y=Par1*EXP(-Par2*X)

Parameters

Par1=R maximum amount bound
Par2=K_-1 dissociation rate constant

Parameter limits

Minimum=-10⁺³⁰
Maximum=10⁺³⁰

Data entry

Enter time values in first column, bound values in the total bound column(s) and unspecific values in the unspecific bound column(s).

Initial estimates

Can be generated automatically or entered manually. Automatic estimates are obtained by linear transformation.