Formula
Y=(Par1/((1+(X/Par2)^Par3)))+Par4
Parameters
Par1=B0 amount bound in the absence of competing drug
Par2=IC50 amount of competing drug inhibiting 50% of labeled binding
Par3=nH pseudo Hill coefficient
Par4=NSB unspecific binding
Parameter limits
Minimum=10-30
Maximum=10+30
Data entry
Enter displacer concentrations in first column and bound values in the total bound column(s). Unspecific binding is calculated by the model
Initial estimates
Can be generated automatically or entered manually. Automatic estimates are obtained by linear transformation and the Hill coefficient is assumed to be 1.