Competition 3 sites

Formula

Y=Par1*Par2/(Par2+X)+Par3*Par4/(Par4+X)+Par5*Par6/(Par6+X)+Par7

Parameters

Par1=R1 amount bound at site 1
Par2=K1 affinity constant for site 1
Par3=R2 amount bound at site 2
Par4=K2 affinity constant for site 2
Par5=R3 amount bound at site 3
Par6=K3 affinity constant for site 3
Par7=NSB unspecific binding

Parameter limits

Minimum=10-30
Maximum=10+30

Data entry

Enter displacer concentrations in first column and bound values in the total bound column(s). Unspecific binding is calculated by the model

Initial estimates

Must be entered manually.