Saturation 1 site with Hill (stimulation)

Formula

Y=(Par1/(1+(Par2/Xval)^Par3))+Par4

Parameters

Par1=Bmax maximum number of binding sites
Par2=EC50 concentration giving 50 % stimulation
Par3=nH Hill coefficient
Par4=E0 amount bound in the absence of stimulating drug

Parameter limits

Minimum=10-30
Maximum=10+30

Data entry

Enter free ligand concentrations in first column, bound values in the total bound column(s) and unspecific values in the unspecific bound column(s).

Initial estimates

Can be generated automatically or entered manually. Automatic estimates are obtained by linear transformation.

(This model is only available in EasyBound for Excel 2000 for Windows)

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