Saturation 2 sites

Formula

Y=Par1*X/(Par2+X)+Par3*X/(Par4+X)

Parameters

Par1=RH amount bound at high affinity site
Par2=KH affinity constant for high affinity site
Par3=RL amount bound at low affinity site
Par4=KL affinity constant for low affinity site

Parameter limits

Minimum=10-30
Maximum=10+30

Data entry

Enter free ligand concentrations in first column, bound values in the total bound column(s) and unspecific values in the unspecific bound column(s).

Initial estimates

Can be generated automatically or entered manually. Automatic estimates are obtained by linear transformation.

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