Saturation 3 sites

Formula

Y=Par1*X/(Par2+X)+Par3*X/(Par4+X)+Par5*X/(Par6+X)

Parameters

Par1=R1 amount bound at site 1
Par2=K1 affinity constant for site 1
Par3=R2 amount bound at site 2
Par4=K2 affinity constant for site 2
Par5=R3 amount bound at site 3
Par6=K3 affinity constant for site 3

Parameter limits

Minimum=10-30
Maximum=10+30

Data entry

Enter free ligand concentrations in first column, bound values in the total bound column(s) and unspecific values in the unspecific bound column(s).

Initial estimates

Must be entered manually.