Saturation 1 site

Formula

Y=Par1*X/(Par2+X)

Parameters

Par1=B_max maximum number of binding sites
Par2=K_d dissociation constant

Parameter limits

Minimum=10^-30
Maximum=10⁺³⁰

Data entry

Enter free ligand concentrations in first column, bound values in the total bound column(s) and unspecific values in the unspecific bound column(s).

Initial estimates

Can be generated automatically or entered manually. Automatic estimates are obtained by linear transformation.