Saturation 2 sites

Formula

Y=Par1*X/(Par2+X)+Par3*X/(Par4+X)

Parameters

Par1=R_H amount bound at high affinity site
Par2=K_H affinity constant for high affinity site
Par3=R_L amount bound at low affinity site
Par4=K_L affinity constant for low affinity site

Parameter limits

Minimum=10^-30
Maximum=10⁺³⁰

Data entry

Enter free ligand concentrations in first column, bound values in the total bound column(s) and unspecific values in the unspecific bound column(s).

Initial estimates

Can be generated automatically or entered manually. Automatic estimates are obtained by linear transformation.