Saturation 3 sites
Formula
Y=Par1*X/(Par2+X)+Par3*X/(Par4+X)+Par5*X/(Par6+X)
Parameters
Par1=R1 amount bound at site 1
Par2=K1 affinity constant for site 1
Par3=R2 amount bound at site 2
Par4=K2 affinity constant for site 2
Par5=R3 amount bound at site 3
Par6=K3 affinity constant for site 3
Parameter limits
Minimum=10-30
Maximum=10+30
Data entry
Enter free ligand concentrations in first column, bound values in the total
bound column(s) and unspecific values in the unspecific bound column(s).
Initial estimates
Must be entered manually.
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